Established in 1800, the Port Health Organisation (PHO), Mumbai serves as the designated public health authority at Mumbai Seaport overseeing the implementation of public health measures for international travellers in accordance with national and international health regulations.

The origins of the organisation date back to 1800, when port quarantine measures were introduced at Mumbai Port to prevent the importation of plague from the Persian Gulf region. Port Surgeons were entrusted with inspecting the health status of passengers and crew arriving on vessels from infected or suspected ports. In 1832, the first set of quarantine rules and regulations for the Bombay Presidency was promulgated. Subsequently, in 1884, the British Government created the full-time post of Health Officer of the Port, formally establishing the Port Health Organisation, Mumbai. Following India's independence, the organisation came under the Government of India. Since 1964, PHO Mumbai has functioned from its present office-cum-residential premises, known as “Pattan Swasthya Bhavan.”

To strengthen public health preparedness and surveillance, PHO Mumbai is equipped with a dedicated vector surveillance laboratory and facilities for training, conferences, and capacity-building activities.

PHO Mumbai is also an authorized Yellow Fever Vaccination Centre.

Additionally, PHO Mumbai oversees and coordinates the public health activities of OTMPs at Maharashtra.